Journal: Gut and Liver

Article Title: The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury

doi: 10.5009/gnl18235

Figure Lengend Snippet: Hepatic differentiation ability in two-dimensional (2D) culture. (A) Gene expression of hepatic differentiation markers (AFP, ALB, and CK18) in transfected ADSCs after 2 weeks measured by qRT-PCR. (B) Amount of cytoplasmic glycogen after the overexpression of various gene in 2D culture measured by periodic acid-Schiff (PAS) staining (PAS staining: blue, nuclei; purple, glycogen in cytoplasm; magnification: ×400). The arrows indicate cytosol. AFP, alpha fetoprotein, ALB, albumin; CK18, cytokeratin 18; ADSCs, adipose tissue-derived stem cells; qRT-PCR, real-time quantitative polymerase chain reaction; Foxa2, forkhead box protein. *p<0.05.

Article Snippet: For liver function evaluation, periodic acid-Schiff staining (PAS Staining Kit, 1.01646.0001; Merck Millipore) was performed to analyze glycogen storage capacity.

Techniques: Gene Expression, Transfection, Quantitative RT-PCR, Over Expression, Staining, Derivative Assay, Real-time Polymerase Chain Reaction

Journal: Gut and Liver

Article Title: The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury

doi: 10.5009/gnl18235

Figure Lengend Snippet: Comparison of hepatic differentiation ability in three-dimensional (3D) culture using a scaffold. (A) qRT-PCR expression of hepatic differentiation markers (AFP, CK18, CD26, and CX32) in Foxa2-transfected ADSCs after 2 weeks in 3D culture. (B) H&E staining, periodic acid-Schiff (PAS) and cytochrome P450 immunofluorescence in Foxa2-overexpressing ADSCs in 3D culture (a and b: H&E staining, blue - nuclei, pink - cytosol, ×400; c and d: PAS staining, blue - nuclei, purple - glycogen in cytoplasm, ×1,000; e and f: blue - nuclei, green - cytochrome 450 in cytosol, ×1,000). (C) Quantification of cytochrome P450 intensity and glucose expression. qRT-PCR, real-time quantitative polymerase chain reaction; AFP, alpha fetoprotein; CK18, cytokeratin 18; CD26, dipeptidyl peptidase 4; CX32, connexin 32; ADSCs, adipose tissue-derived stem cells; Foxa2, forkhead box protein. *p<0.05.

Article Snippet: For liver function evaluation, periodic acid-Schiff staining (PAS Staining Kit, 1.01646.0001; Merck Millipore) was performed to analyze glycogen storage capacity.

Techniques: Comparison, Quantitative RT-PCR, Expressing, Transfection, Staining, Immunofluorescence, Real-time Polymerase Chain Reaction, Derivative Assay

Journal: Gut and Liver

Article Title: The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury

doi: 10.5009/gnl18235

Figure Lengend Snippet: Comparison of hepatocyte differentiation in a scaffold during in vivo culture. (A) In vivo culture design of scaffold preparation and implantation into nude mice. (B) Scanning electron microscopy of ADSCs in the PLGA scaffold. (C) qRT-PCR expression of hepatic differentiation markers (AFP, CK18, CD26 and CX32) in Foxa2-transfected ADSCs after 2 weeks in nude mice. (D) H&E staining, periodic acid-Schiff (PAS) staining and immunofluorescent staining for cytochrome P450 activity after Foxa2 gene overexpression in in vivo culture (a and b: H&E staining, blue - nuclei, pink - cytosol, ×400; c and d: PAS staining, blue - nuclei, purple - glycogen in cytoplasm, ×1,000; e and f: blue - nuclei, green - cytochrome 450 in cytosol, ×1,000). (E) Quantification of cytochrome P450 intensity and glucose expression. ADSCs, adipose tissue-derived stem cells; PLGA, poly(lactic-co-glycolic acid); qRT-PCR, real-time quantitative polymerase chain reaction; AFP, alpha fetoprotein; CK18, cytokeratin 18; CD26, dipeptidyl peptidase 4; CX32, connexin 32; Foxa2, forkhead box protein. *p<0.05.

Article Snippet: For liver function evaluation, periodic acid-Schiff staining (PAS Staining Kit, 1.01646.0001; Merck Millipore) was performed to analyze glycogen storage capacity.

Techniques: Comparison, In Vivo, Electron Microscopy, Quantitative RT-PCR, Expressing, Transfection, Staining, Activity Assay, Over Expression, Derivative Assay, Real-time Polymerase Chain Reaction

Journal: Gut and Liver

Article Title: The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury

doi: 10.5009/gnl18235

Figure Lengend Snippet: Stem cell homing. (A) Mouse and human β2-microglobulin mRNA expression was compared between the TAA-only group and the ADSCs scaffold group. (B) Immunofluorescence stain using human anti-mitochondria antibody was performed in mouse liver (×200) and implanted scaffold (×630) (immunofluorescence: blue - nuclei, green - human mitochondria). H&E staining (blue - nuclei, pink - cytosol, ×400) and periodic acid-Schiff (PAS) staining (blue - nuclei, purple - glycogen in cytoplasm, ×630) of implanted scaffold. TAA, thioacetamide; ADSCs, adipose tissue-derived stem cells; Foxa2, forkhead box protein.

Article Snippet: For liver function evaluation, periodic acid-Schiff staining (PAS Staining Kit, 1.01646.0001; Merck Millipore) was performed to analyze glycogen storage capacity.

Techniques: Expressing, Immunofluorescence, Staining, Derivative Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Apigenin attenuates PM2.5-induced airway hyperresponsiveness and inflammation by down-regulating NF-κB in murine model of asthma

doi:

Figure Lengend Snippet: Treatment with apigenin attenuated mucus production in a murine model of asthma exposed to PM2.5. PAS staining (×200) of: (A) Comtrol group; (B) OVA group; (C) PM2.5 group; (D) Apigenin group; (E) Dexamethasone group. (F) The percentage of PAS-positive cells per bronchiole. Data are the means ± SEM (n=6). *P < 0.05, compared with the control group. ▲P < 0.05, compared with the OVA group. ■P < 0.05, compared with the PM2.5 group.

Article Snippet: PAS staining (×200) of: (A) Comtrol group; (B) OVA group; (C) PM2.5 group; (D) Apigenin group; (E) Dexamethasone group. (F) The percentage of PAS-positive cells per bronchiole.

Techniques: Staining, Control